Mrs. Killer is the main antagonist in the 1995 film Tonia. She is a red anthropomorphic crayon and Tonia's enemy.

Slappy and his crew went on a boat to go to the jungle. With the introduction of Tonia, Mrs. Killer makes pictures slides on to let Tonia and friends see them. At night, a driver takes Mrs. Killer and her crew to the movie theater. After the theater, Mrs. Killer and Tonia share a moment. She manipulates the rabbit into believing to stay with her friends. In the jungle, Mrs. Killer tries to kill Freddy and Melvin, who are dressed at spies. Tonia, however is shocked and has Kristian, the brown bear to distract. Kristian returns angrily and charges at Mrs. Killer, who tries to kill Tonia's friends, but Tonia stops him from doing it. Disowned, Kristian was out of control, Mrs. Killer storms off, leaving Tonia to be shocked. In the lair, Mrs. Killer explains to the crew that they will destroy Tonia and her friends. Mrs. Killer's crew capture the animals. Kristian attempted to rescue. Mrs. Killer slowly approaches the bear and prepares to kill him. Tonia kicks the crayon to the ground. She saves Kristian, who was captured. From the jungle, Mrs. Killer smacks Tonia in the face. Before Mrs. Killer tries to kill her, Kristian sees this and charges toward her, but Mrs. Killer turns and overpowers Kristian with a crowbar. Tonia and Mrs. Killer fight in the jungle. They exchange blows. When nature wins, Tonia points the sword at Mrs. Killer. Mrs. Killer says her last words, " If you and your friends won't be dead, I will be the friend of you." and pulls out her kukri, then tries to kill the bunny. Tonia jumped back into a mass of vine to escape Mrs. Killer's furious swipes. She manages to tie Mrs. Killer with the vine, but Mrs. Killer mindlessly hacked at them with her kukri. Tonia tries to warn her, but in her rage, Mrs. Killer accidentally cut the vine she was holding to, causing her and Tonia to plummet toward the ground. Tonia landed safely, but Mrs. Killer was hanged by the vine and died when it snapped her neck. A flash of lightning illuminated the shadow of her hanged corpse.